多重PCR方法快速鉴别肠炎、鼠伤寒、鸡白痢及鸡伤寒沙门菌

doi:10.11843/j.issn.0366-6964.2014.02.015

Abstract

Abstract:

Based on the sequences of the hut gene specific for Salmonella and the SdfⅠ, Spy，glgC，and Spec genes specific for Salmonella serotypes，five pairs of primers were designed and synthesized.A multiplex PCR (mPCR) method was developed and uses for detection of clinical Salmonella isolates.The results showed that the strains of S.enteritidis，S.typhimurium，S.pullorum and S.gallinarum were identified specifically by the mPCR，the sensitivities of mPCR for all bacteria were 3×10³ CFU，and the sensitivities of mPCR for chromosomal DNA of S.enteritidis，S.typhimurium，S.pullorum and S.gallinarum were 162，202，214 and 178 pg·μL^－1，respectively.Compared to the traditional serological assay，the sensitivities of the PCR for 55 Salmonella clinical isolates were 100%，and the minimal coincidence rate of the mPCR assay were 96.4%.The results indicated that the mPCR assay is a rapid，sensitive and specific method for identification of the four serotypes Salmonella.

CLC Number:

S852.612

YANG Lin，LOU Ya-kun，SU Chun-hu，ZHANG Hua，GUAN Meng，XU Chen-xu，CHEN Su-juan，WEI Rong，CHEN Ji-ming，PENG Da-xin. Development of a Multiplex PCR for Rapid Identification of Salmonella enteritidis，Salmonella typhimurium，Salmonella pullorum and Salmonella gallinarum[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(2): 268-273.

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Development of a Multiplex PCR for Rapid Identification of Salmonella enteritidis，Salmonella typhimurium，Salmonella pullorum and Salmonella gallinarum

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